High-throughput sequencing of 16S rRNA Gene Reveals Substantial Bacterial Diversity on the Municipal Dumpsite
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چکیده
منابع مشابه
Bacterial Community Diversity of Oil-Contaminated Soils Assessed by High Throughput Sequencing of 16S rRNA Genes
Soil bacteria play a major role in ecological and biodegradable function processes in oil-contaminated soils. Here, we assessed the bacterial diversity and changes therein in oil-contaminated soils exposed to different periods of oil pollution using 454 pyrosequencing of 16S rRNA genes. No less than 24,953 valid reads and 6246 operational taxonomic units (OTUs) were obtained from all five studi...
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in this study, to increase information about the relationship between caprine and ovine isolates of pasteurella multocida, 16s rrna gene sequencing of 9 goats (5) and sheep (4) isolates were investigated. also, capsular type and toxagene presentation was studied in this paper. all isolates, except one, belong to capsular type a, and toxa+ strain distributed among strains were isolated from both...
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The stratified distribution of bacterial and archaeal communities has been detected in many sediment profiles from various natural environments. A better understanding of microbial composition and diversity pattern in coastal mangrove wetlands in relation to physicochemical and spatial-temporal influences could provide more insights into the ecological functions of microbes in coastal wetlands....
متن کاملSynthetic spike-in standards for high-throughput 16S rRNA gene amplicon sequencing
High-throughput sequencing of 16S rRNA gene amplicons (16S-seq) has become a widely deployed method for profiling complex microbial communities but technical pitfalls related to data reliability and quantification remain to be fully addressed. In this work, we have developed and implemented a set of synthetic 16S rRNA genes to serve as universal spike-in standards for 16S-seq experiments. The s...
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ژورنال
عنوان ژورنال: BMC Microbiology
سال: 2016
ISSN: 1471-2180
DOI: 10.1186/s12866-016-0758-8